In plants: (1986-1988) Prof. Huffacker of UCD, Davis USA, tested AMINOL-FORTE containing "FACE" as an active material, in wheat plants at doses 1,000 times higer than standard (0.1%), no toxic or physiological harmful effects were observed.
In animals: (1990-1991) Life Science Research, Ltd., have tested "FACE" under the code name "ULC-87" in mice, rats and beagle dogs.

No effects were observed. Oral administration to CD rats and beagles dogs, 2,500mg/Kg/day and 4,000mg/Kg/day, did not provide evidence of toxicity.

(1992) BIOGIR S.A., in albino guinea pigs (Magnesson and Kligman test), ALEC-28 (FACE) was considered as hypoallergenic.

In rabbit, assessment of cutaneous tolerance showed no effect of irritability to skin.
In humans: (1992) Under the code name ALEC-28 as a cream, FACE was tested by BIOGIR, S.A., Burdeaux, France, in dermatological applications. In the cutaneous tolerance test on clinical cases of allergic pathology FACE showed that it did not induce any cutaneous primary irritacy and proved to not irritate the eyes.
In cells: (1988-1990) In in-vitro cell primary cultures of adult male rat cerebrocortical neuroblasts cells, no toxicological effects were observed. On the contrary significant positive neurotrophic effects were observed and measured. Hospital of Ramon y Cajal. Departament of Neuro-physiology Madrid Spain and Carlos III National Health Institute Madrid Spain.
(1992) Contox S.A.,: "neoplasia test" in eucaryotic cell cultures (BHK-21-C13): No toxic or genotoxic effects were observed with FACE.


Toxicological tests on the biologically active "FACE" also identified by the code names "ULC-87", "NOMAR-200", "ALEC-28" show:

In Vitro: No damage, toxicity nor geno-toxicity in cells were observed.
In Vivo: In tests with oral, intramuscular, intravenous administration to mice, rats and beagle dogs, under internacionally regulated standard protocols for the testing of toxicological effects, no effects were observed, thus classifying the substance as having "low and very low toxicity"

Oral administration of doses up to 4,000mg/Kg, showed no effects on organs (brain, liver, spleen, etc.), no death of animals was produced. LD50 determinations of acute oral and intravenous in mice and rats was greater than 2000mg/Kg. No real LD50 figure could be determined no deaths in the animals was reported under the conditions of the test.